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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
27/01/2023 |
Data da última atualização: |
24/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MACHADO-ROSA, T. A.; BARBOSA, E. T.; CÔRTES, M. V. de C. B.; LOBO JUNIOR, M. |
Afiliação: |
TARIANE A. MACHADO-ROSA, UNIVERSIDADE FEDERAL DE GOIÁS; ELDER TADEU BARBOSA, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MURILLO LOBO JUNIOR, CNPAF. |
Título: |
Microtiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Rhizosphere, v. 25, 100666, 2023. |
ISSN: |
2452-2198 |
DOI: |
https://doi.org/10.1016/j.rhisph.2023.100666 |
Idioma: |
Inglês |
Conteúdo: |
A quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 μL of the super-natant were added to 100 μL of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method. |
Palavras-Chave: |
Biocontrol; Plant growth promotion; Stepwise screening. |
Thesagro: |
Estimulante de Crescimento Vegetal; Fungo; Trichoderma. |
Thesaurus Nal: |
Auxins; Plant growth; Root exudates. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01851naa a2200289 a 4500 001 2151274 005 2023-03-24 008 2023 bl uuuu u00u1 u #d 022 $a2452-2198 024 7 $ahttps://doi.org/10.1016/j.rhisph.2023.100666$2DOI 100 1 $aMACHADO-ROSA, T. A. 245 $aMicrotiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma.$h[electronic resource] 260 $c2023 520 $aA quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 μL of the super-natant were added to 100 μL of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method. 650 $aAuxins 650 $aPlant growth 650 $aRoot exudates 650 $aEstimulante de Crescimento Vegetal 650 $aFungo 650 $aTrichoderma 653 $aBiocontrol 653 $aPlant growth promotion 653 $aStepwise screening 700 1 $aBARBOSA, E. T. 700 1 $aCÔRTES, M. V. de C. B. 700 1 $aLOBO JUNIOR, M. 773 $tRhizosphere$gv. 25, 100666, 2023.
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